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Avian Bioscience Research Center
Nagoya Univ. Graduate School of Bioagricultural Sciences

Chicken strains and lines	Quail lines
Ancestral species Inbred strains Long-term closed colonies Closed colonies Disease models Developing colonies Transgenic chickens Primordial germ cell lines	Long-term closed coloniesTransgenic quails

Transgenic quails

|PGK:H2B-chFP-TG (chPF)|

PGK:H2B-chFP-TG (chPF)

Origin

Transgenic quail expressing a fluorescent protein (chFP) created in the Rusty Lansford lab at the University of Southern California.(Sato et al., 2010, Sato & Lansford, 2013). Introduced from Kyushu University with permission from the creator.
The chFP protein gene used to create this TG quail was a direct gift from its creator, Dr. Roger Tsien (California State University, San Diego).

Characteristics

plumage and colors：	wild-type plumage wild-type colored egg
body weight：	adult male：- g adult female：- g

This line was established by lentivirus vector (PGK1::H2B-chFP expressing) infection into WJ quails. The ChFP expression is observed almost throughout the body. Inserted genes have been confirmed at multiple locations on the genome, and the observed fluorescence intensity varies from each individual.

Reference

Huss et al. 2015. A transgenic quail model that enables dynamic imaging of amniote embryogenesis. Development. 142: 2850-2859
doi: 10.1242/dev.121392

When publihing a paper, please cites the above paper (Huss et al. Development 2015) and includes the names of Rusty Lansford and Yuki Sato in the acknowledgments.

○Appication for live imaging
Yoshihi et al. 2022. “Live imaging of avian epiblast and anterior mesendoderm grafting reveals the complexity of cell dynamics during early brain development." Development 149 (6): dev199999.

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