Chicken strains and lines	Quail lines
Ancestral speciesInbred strainsLong-term closed coloniesClosed colonies Disease modelsDeveloping coloniesTransgenic chickens Primordial germ cell lines	Long-term closed coloniesTransgenic quails

Transgenic chickens

｜ pLsi/ΔAeGFP ｜ PRDM14-egfp ｜ Cas9-T2A-mCherry/PB ｜

pLSi/ΔAeGFP

Origin

This line is the transgenic chicken expressing eGFP under chicken actin promoter which created by Shinji Iijima's laboratory in the Department of Chemical and Biological Engineering, Nagoya Graduate School of Engineering (Motono et al., 2010)。 。

Characteristics

The EGFP gene expressing lentivirus vector infected PGCs which collected from Nisseiken White Leghorn (LINE-M) were transplanted into HH st.13~15 LIME-M embryos, and this line was established from mating by these germ -line chimeric G0 chickens.The EGFP gene is controlled under chicken beta-actin promoter and ubiquitous EGFP expression is observed in whole embryos. Carrier populations are currently maintained by crossing with the WL-M/O strain.

References

Motono et al. 2010. Production of transgenic chickens from purified primordial germ cells infected with a lentiviral vector. J Biosci Bioeng. 109(4):315–321. doi:10.1016/j.jbiosc.2009.10.007

Tsujino et al. 2019. Identification of transgene integration site and anatomical properties of fluorescence intensity in a EGFP transgenic chicken line. Dev. Growth. Differ. 61:393–401. doi:10.1111/dgd.12631

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PRDM14^egfp (PRDM14-knockin/knockout chicken)

Origin

This line is the transgenic chicken that specifically expresses fluorescent protein (eGFP) in primordial germ cells which created in Kenichi Nishijima's laboratory in the Department of Chemical and Biological Engineering, Nagoya Graduate School of Engineering.

Characteristics

The EGFP gene was knocked in PRDM14 loci of in vitro cultured PGCs (WL-M/O) using CRISPR-Cas9 and those were transplanted into HH st.13~15 LIME-M embryos, then this line was established from mating by these germ-line chimeric G0 chickens. The EGFP expression is controlled under endogenous PRDM14 promoter and is observed in blood circulating and gonadal PGCs. In adults, EGFP expression is observed in the testis. PRDM14egfp/egfp shows early stage embryonic lethality.

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Cas9-T2A-mCherry/PB

Origin

This line is the transgenic chicken expressing Streptococcus pyogenes Cas9 (SpCas9) created by Kenichi Nishijima's laboratory at the Department of Animal Science, Graduate School of Bioagricultural Sciences, Nagoya University.

Characteristics

The SpCas9-T2A-mCherry expression cassette was introduced in cultured PGCs (WL-M/O) using PiggyBac transposase and those were transplanted into HH st.13~15 Julia light embryos, then this line was established from mating by these germ-line chimeric G0 chickens. The SpCas9/mCherry expression was controlled under human EF1-alpha promoter and is ubiquitously observed, but its intensity is mild. Cas9 activity has been confirmed by infecting sgRNA expressing adeno associated vector into blastoderm.

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