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【論文等】

2024年

プレプリント

Usui, K., Yamamoto, H., Mori, H. and Fujita, Y. (2024) Extracellular vesicle-mediated secretion of chlorophyll biosynthetic intermediates in the cyanobacterium Leptolyngbya boryana. BioRxiv, https://www.biorxiv.org/content/10.1101/2024.03.29.587320v1

原著論文

Uesaka, K., Banba, M., Chiba, S., and Fujita, Y. (2024) Restoration of the functional nif gene cluster by seven excision and two inversion events during heterocyst development in the nitrogen-fixing cyanobacterium Calothrix sp. NIES-4101. Plant Cell Physiol, doi:https://doi.org/10.1093/pcp/pcae011

2023年

プレプリント

Uesaka, K., Banba, M., Chiba, S., and Fujita, Y. (2023) Restoration of the functional nif gene cluster by seven excision and two inversion events during heterocyst development in the nitrogen-fixing cyanobacterium Calothrix sp. NIES-4101. BioRxiv, https://www.biorxiv.org/content/10.1101/2023.12.11.571196v1

原著論文

Toida, K., Yamamoto, H., Yamamoto, K., Ishii, K., Uesaka, K., Kanaly, R.A., Kutsuna, S., Ihara, K., Fujita, Y., and Iwasaki, H. (2023) The GGDEF protein Dgc2 suppresses both mobility and biofilm formation in the filamentous cyanobacterium Leptolyngbya boryana. Microbiol. Spectrum, https://doi.org/10.1128/spectrum.04837-22

2022年

原著論文

Yamamoto, H., Uesaka, K., Tsuzuki, Y., Yamakawa, H., Itoh, S., and Fujita, Y. (2022) Comparative genomic analysis of the marine cyanobacterium Acaryochloris marina MBIC10699 reveals the impact of phycobiliprotein reaquisition and the diversity of Acaryochloris plasmids. Microorganisms, 10, 1374, DOI: 10.3390/microorganisms10071374

Tsuzuki, Y., Tsukatani, Y., Yamakawa, H., Itoh, S., Fujita, Y., and Yamamoto, H. (2022) Effects of light and oxygen on chlorophyll d biosynthesis in a marine cyanobacterium Acaryochloris marina. Plants, 11, 915, DOI: 10.3390/plants11070915

Usui, K., Yamamoto, H., Oi, T., Taniguchi, M., Mori, H., and Fujita, Y. (2022) Extracellular vesicle-mediated secretion of protochlorophyllide in the cyanobacterium Leptolyngbya boryana. Plants, 11, 910, DOI: 10.3390/plants11070910

総説

Fujita, Y. and Uesaka, K. (2022) Nitrogen fixation in cyanobacteria. Cyanobacterial Physiology: From Fundamentals to Biotechnology, Kageyama, H. and Waditee-Sirisattha, R. eds., Academic Press, pp. 29-45

2020年

原著論文

Yamamoto, H., Mizoguchi, T., Tsukatani, Y., Tamiaki, H., Kurisu, G., and Fujita, Y. (2020) Chlorophyllide a oxidoreductase preferentially catalyzes 8-vinyl reduction over B-ring reduction of 8-vinyl chlorophyllide a in the late steps of bacteriochlorophyll biosynthesis. ChemBioChem, 21, 1760-1766, DOI: 10.1002/cbic.201900785

Yamamoto, H., Kojima-Ando, H., Ohki, K., and Fujita, Y. (2020) Formation of prolamellar-body-like ultrastructures in etiolated cyanobacterial cells overexpressing light-dependent protochlorophyllide oxidoreductase in Leptolyngbya boryana. J. Gen. Appl. Microbiol., 66, 129-139, DOI: 10.2323/jgam.2020.01.009

2019年

原著論文

Hiraide, Y, Yamamoto, H., Kawajiri, Y., Yamakawa, H., Wade, K., and Fujita, Y. (2019) Super-activator variants of the cyanobacterial transcriptional regulator ChlR essential for tetrapyrrole biosynthesis under low oxygen conditions. Biosci. Biotechnol. Biochem., in press, DOI: 10.1080/09168451.2019.1687281

Nonaka, A., Yamamoto, H., Kamiya, N., Kotani, H., Yamakawa, H., Tsujimoto, R., and Fujita, Y. (2019) Accessory proteins of the nitrogenase assembly, NifW, NifX/NafY, and NifZ, are essential for diazotrophic growth in the nonheterocystous cyanobacterium Leptolyngbya boryana. Front. Microbiol., 10, 495, DOI: 10.3389/fmicb.2019.00495

2018年

原著論文

Ryo, M., Yamashino, T., Yamakawa, H., Fujita, Y., and Aoki, S. (2018) PAS-histidine kinases PHK1 and PHK2 exert oxygen-dependent dual and opposite effects on gametophore formation in the moss Physcomitrella patens. Biochem. Biophys. Res. Commun., 503, 2861-2865, DOI: 10.1016/j.bbrc.2018.08.056

Tomatsu, C., Uesaka, K., Yamakawa, H., Tsuchiya, T., Ihara, K., and Fujita, Y. (2018) In vivo transposon tagging in the nonheterocystous cyanobacterium Leptolyngbya boryana. FEBS Lett., 592, 1634-1642, DOI: 10.1002/1873-3468.13079

Tsujimoto, R., Kotani, H., Yokomizo, K., Yamakawa, H., Nonaka, A., and Fujita, Y. (2018) Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism. Sci. Rep., 8, 7380, DOI: 10.1038/s41598-018-25396-7

総説

藤田祐一、山川壽伯、山本治樹 (2018) 光合成生物に窒素固定酵素の活性を付与する〜空気を肥料とする植物の作出を目指して〜 化学 73,(11) 29-33

2017年

原著論文

Yamamoto, H., Kusumi, J., Yamakawa, H., and Fujita, Y. (2017) The effect of two amino acid residue substitutions via RNA editing on dark-operative protochlorophyllide oxidoreductase in the black pine chloroplasts. Sci. Rep., 7, 2377, DOI: 10.1038/S41598-017-02630-2

Terauchi, K., Sobue, R., Furutani, Y., Aoki, R., and Fujita, Y. (2017) Isolation of cyanobacterial mutants exhibiting growth defects under microoxic conditions by transposon mutagenesis of Synechocystis sp. PCC 6803. J. Gen. Appl. Microbiol., 63, 131-138

総説

水野猛、山篠貴史 (2017) 植物の概日時計と和時計〜植物に内在する時計のしくみ〜 化学と生物 55, 174-181

著書

Fujita, Y. and Yamakawa, H. (2017) Biochemistry of chlorophyll biosynthesis in photosynthetic prokaryotes. In Modern Topics in Photosynthetic Prokaryotes – Metabolism, Bioenergetics, and Omics, Hallenbeck, P. ed. pp. 67-122, Springer

2016年

原著論文

Tsujimoto, R., Kamiya, N., and Fujita, Y. (2016) Identification of a cis-element in nitrogen fixation genes recognized by CnfR in the nonheterocystous nitrogen-fixing cyanobacterium Leptolyngbya boryana. Mol. Microbiol. 101, 411-424, DOI: 10.1111/mmi.13402.

Nomata, J., Terauchi, K. and Fujita, Y. (2016) Stoichiometry of ATP hydrolysis and chlorophyllide formation of dark-operative protochlorophyllide oxidoreductase from Rhodobacter capsulatus. Biochem. Biophys. Res. Commun., 470, 704-709, DOI:10.1016/j.bbrc.2016.01.070.

総説

藤田祐一、辻本良真 (2016) 酸素パラドクスの統御で収斂するクロロフィル生合成と窒素固定. 光合成研究 26, 204-215

Ryo, M., Matsuo, T., Yamashino, T., Ichinose, M., Sugita, M. and Aoki, S. (2016) Diversity of plant circadian clocks: Insights from studies of Chlamydomonas reinhardtii and Physcomitrella patens. Plant Signal. Behav. 11, DOI: 10.1080/15592324.2015.1116661.

2015年

原著論文

Tsujimoto, R., Kotani, H., Nonaka, A., Miyahara, Y., Hiraide, Y. and Fujita, Y. (2015) Transformation of the cyanobacterium Leptolyngbya boryana by electroporation. Bio-protocol, 5, 24, e1690.

Mizuno, T., Katayama, M., Takayama, C. and Yamashino, T. (2015) Insight into a physiological role for the EC night-time repressor in the Arabidopsis circadian clock. Plant Cell Physiol. 56, 1738-1747. DOI: 10.1093/pcp/pcv094.

Mizuno, T. and Yamashino, T. (2015) The plant circadian clock looks like a traditional Japanese clock rather than a modern Western clock. Plant Signal. Behav. 10, DOI: 10.1080/ 15592324.2015.1087630.

Yamanashi, K., Minamizaki, K. and Fujita, Y. (2015) Identification of the chlE gene encoding oxygen-independent Mg-protoporphyrin IX monomethyl ester cyclase in cyanobacteria. Biochem. Biophys. Res. Commun. 463, 1328-1333; DOI: 10.1016/j.bbrc.2015.06.124

Tsukatani, Y., Harada, J., Nomata, J., Yamamoto, H., Fujita, Y., Mizoguchi, T., Tamiaki, H. (2015) Rhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthesis pathway. Sci. Rep. 5, 9741; DOI:10.1038/srep09741

Mizuno, T., Oka, H., Yoshimura, F., Ishida, K. and Yamashino, T. (2015) Insight into the mechanism of end-of day far-red light (EODFR)-induced shade avoidance response in Arabidopsis thaliana. Biosci. Biotech. Biochem. 79, 1987-1994.

Hiraide, Y., Oshima, K., Fujisawa, T., Uesaka, K., Hirose, Y., Tsujimoto, R., Yamamoto, H., Okamoto, S., Nakamura, Y., Terauchi, K., Omata, T., Ihara, K., Hattori, M. and Fujita, Y. (2015) Loss of cytochrome cM stimulates cyanobacterial heterotrophic growth in the dark. Plant Cell Physiol., 56, 334-345, DOI:10.1093/pcp/pcu165. (PCP, Research Highlightsで紹介)

総説

Fujita, Y., Tsujimoto, R. and Aoki, R. (2015) Evolutionary aspects and regulation of tetrapyrrole biosynthesis in cyanobacteria under aerobic and anaerobic environments. Life, 5, 1172-1203; DOI:10.3390/life5021172

藤田祐一・山本治樹 (2015) 第21章 窒素の固定 「光合成のエネルギー変換と物質変換」pp.215-219、化学同人

Fujita, Y. (2015) Chlorophylls (ver. 3.0) In Encyclopedia of Life Sciences,(3rd edition) John Wiley & Sons, Ltd: Chichester, doi: 1002/9780470015902.a0000661.pub3

その他

藤田祐一 (2015) 光合成辞典 日本光合成研究会編 http://photosyn.jp/pwiki/index.php?光合成事典 (14項目「クロロフィリドa」「クロロフィリドaレダクターゼ」「クロロフィルシンターゼ」「クロロフィルaの生合成」「ニトロゲナーゼ類似酵素」「光非依存型プロトクロロフィリドレダクターゼ」「プロトクロロフィリド」「マグネシウムプロトポルフィリンIXメチルトランスフェラーゼ」「シキミ酸経路」「5-エノールピルビルシキミ酸3-リン酸シンターゼ」「ニトロゲナーゼ」「鉄硫黄クラスター」「フェレドキシン」「非ヘム鉄」)

2014年

原著論文

Mizuno, T., Kitayama, M., Oka, H., Tsubouchi, M., Takayama, C., Nomoto, Y. and Yamashino, T. (2014) The EC night-time repressor plays a crucial role in modulating circadian clock transcriptional circuitry by conservatively double-checking both warm-night and night-time-light signals in a synergistic manner in Arabidopsis thaliana. Plant Cell Physiol. 55, 2139-2151; DOI: 10.1093/pcp/pcu144.

Nomata, J., Kondo, T., Mizoguchi, T., Tamiaki, H., Itoh, S. and Fujita, Y. (2014) Dark-operative protochlorophyllide oxidoreductase generates substrate radicals by an iron-sulphur cluster in bacteriochlorophyll biosynthesis. Sci. Rep. 4, 5455; DOI:10.1038/srep05455.

Yamamoto, H., Kato, M., Yamanashi, K., and Fujita, Y. (2014) Reconstitution of a sequential reaction of two nitrogenase-like enzymes in the bacteriochlorophyll biosynthetic pathways of Rhodobacter capsulatus. Biochem. Biophys. Res. Commun. 448, 200-205.

Tsujimoto, R., Kamiya, N., and Fujita, Y. (2014) Transcriptional regulators ChlR and CnfR are essential for diazotrophic growth of nonheterocystous cyanobacteria. Proc. Natl. Acad. Sci. USA 111, 6762-6767.

Mizuno, T., Nomoto, Y., Oka, H., Kitayama, M., Takeuchi, A., Tsubouchi, M. and Yamashino, T. (2014) Ambient temperature signal feeds into the circadian clock transcriptional circuitry through the EC night-time repressor in Arabidopsis thaliana. Plant Cell Pysiol. 55, 958-976; DOI:10.1093/pcp/pcu030.

Mizuno, T., Takeuchi, A., Nomoto, Y., Nakamichi, N., and Yamashino, T. (2014) The LNK1 night light-inducible and clock-regulated gene is induced also in response to warm-night through the circadian clock nighttime repressor in Arabidopsis thaliana. Plant Signal. Behav. 9, e28505.

Aoki, R., Hiraide, Y., Yamakawa, H. and Fujita, Y. (2014) A novel “oxygen-induced” greening process in a cyanobacterial mutant lacking the transcriptional activator ChlR involved in low-oxygen adaptation of tetrapyrrole biosynthesis. J. Biol. Chem. 289, 1841-1851.


【主要講演】

2023年

US-Japan Binational Photosynthesis Workshop(Arizona State University)November 6-8, 2023(招待講演)
Yuichi Fujita, Mutations promoting dark heterotrophic growth and suppressing photosynthetic growth in the cyanobacterium Leptolyngbya boryana

2019年

5th Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation(東北大学)May 16, 2019(招待講演)
Yuichi Fujita, Enhancement of nitrogenase activity in transform ants of the non-diazotrophic cyanobacterium Synechocystis sp. PCC 6803 carrying the nif genes

2018年

10th International Conference on Porphyrins and Phthalocyanines(ドイツ、ミュンヘン)July 6, 2018(招待講演)
Yuichi Fujita, Assembly of the catalytic component, NB protein, of dark-operative protochlorophyllide oxidoreductase

Stories of Oxygen and Active Molecular Species in Photosynthetic Organisms, 59th Annual Meeting of Japanese Society of Plant Physiologists(札幌)March 28, 2018(招待講演)
Yuichi Fujita, Mechanisms to control the Oxygen Paradox between photosynthesis and nitrogen fixation

2017年

The 20th International Congress on Nitrogen Fixation(スペイン、グラナダ)September 5, 2017(プレナリーレクチャー)
Yuichi Fujita, Characterization of the transcriptional regulator CnfR for then nif genes in nitrogen fixing cyanobacteria

Polytechnic University of Madrid, CBGP Seminar(スペイン、マドリード)March 31, 2017(招待講演)
Yuichi Fujita, Cyanobacterial low oxygen adaptation mechanisms resolving the oxygen paradox between photosynthesis and nitrogen fixation

日本植物生理学会 第19回オルガネラワークショップ(鹿児島大学)2017年3月15日(招待講演)
藤田祐一「光合成と窒素固定の酸素パラドクスを統御する低酸素適応機構」

2016年

第27回フォーラム・イン・ドージン「低酸素応答の光と影~エリスロポエチン純化から40年~」(熊本ホテルキャッスル)2016年10月28日(招待講演)
藤田祐一「光合成と窒素固定の酸素パラドクスの統御機構」

日本光合成学会シンポジウム(東京理科大)2016年5月27日〜28日(招待講演)
藤田祐一「シアノバクテリアの低酸素適応機構と酸素依存的緑化」

農芸化学会2016年度大会シンポジウム「酸素微生物学~微生物に学ぶ『酸素リモデリング』とその応用~」(札幌コンベンションセンタ-)2016年3月29日(招待講演)
藤田祐一「酸素発生型光合成を行うシアノバクテリア(藍藻)の低酸素適応機構」

日本植物生理学会 第1回光合成細菌ワークショップ(岩手大学)2016年3月18日(招待講演)
藤田祐一「クロロフィル生合成研究を先導する光合成細菌Rhodobacter capsulatus

The 5th International Conference on MEXT Project of Integrated Research on Chemical Synthesis “Chemical Science for Future Societies”(名古屋大学)January 29-30, 2016(招待講演)
Yuichi Fujita, Nitrogenase-like reductases in bacteriochlorophyll biosynthesis: Reaction mechanism and plasticity

2015年

第38回分子生物学会・第88回生化学会合同年会 ワークショップ「鉄硫黄タンパク質が織りなす多彩な機能」(神戸ポートアイランド)2015年12月1日(招待講演)
藤田祐一「暗所での緑化を決定づけるニトロゲナーゼ類似酵素」

19th International Congress on Nitrogen Fixation(米国カリフォルニア州、パシフィックグローブ)October 4–9, 2015, Pacific Grove, CA, USA(招待講演)
Yuichi Fujita, Reaction mechanism of a nitrogenase-like protochlorophyllide reductase.

15th International Symposium on Phototrophic Prokaryotes(ドイツ、チュービンゲン)August 2–6, Tübingen, Germany(口頭発表)
Yuto Hiraide, Kazuma Uesaka, Kunio Ihara and Yuichi Fujita, Loss of the cytM gene encoding cytochrome cM stimulates dark heterotrophic growth of cyanobacteria.

第88回日本細菌学会総会 シンポジウム「環境保全とエネルギー開発に貢献する微生物」(長良川国際会議場)2015年3月27日(招待講演)
藤田祐一・小俣達男「光合成微生物による資源・エネルギー供給の展望」

大阪大学蛋白質研究所セミナー「嫌気蛋白質を対象とした構造・機能相関の現状」(大阪大学蛋白質研究所)2015年3月5日(招待講演)
藤田祐一「暗所作動型プロトクロロフィリド還元酵素の反応機構解析」

2014年

3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation(中国、成都)October 28-30, 2-14, 2014, Chengdu, China(招待講演)
Yuichi Fujita, CnfR is a master transcriptional activator essential for nitrogen fixation in nonheterocystous cyanobacteria.

Institute for Protein Research Seminar, Regulation of Environmental Adaptation of Photosynthesis: An Attractive Theme for Structural Life Sciences (大阪大学蛋白質研究所)October 24, 2014, Osaka University, Japan(招待講演)
Yuichi Fujita, Chlorophyll biosynthesis under low oxygen environments.