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1.        New! Terauchi, K., Sobue, R., Furutani, Y., Aoki, R., and Fujita, Y.  (2016) Isolation of cyanobacterial mutants exhibiting growth defects under microoxic conditions by transposon mutangenesis of Synechocystis sp. PCC 6803. J. Gen. Appli. Microbiol, in press.

2.        New! Tsujimoto, R., Kamiya, N., and Fujita, Y.  (2016) Identification of a cis-acting element in nitrogen fixation genes recognized by CnfR in the nonheterocystous nitrogen-fixing cyanobacterium Leptolyngbya boryana. Mol. Microbiol., 101, 411-424, doi:10.1111/mmi.13402

3.        Nomata, J., Terauchi, K., and Fujita, Y.  (2016) Stoichiometry of ATP hydrolysis and chlorophyllide formation of dark-operative protochlorophyllide oxidoreductase from Rhodobacter capsulatus. Biochem. Biophys. Res. Commun., 470, 704-709, doi:10.1016/j.bbrc.2016.01.070

 

4.        Tsujimoto, R., Kotani, H., Nonaka, A., Miyahara, Y, Hiraide, Y. and Fujita, Y.  (2015) Transformation of the cyanobacterium Leptolyngbya boryana by electroporation. Bio-Protocol, 5 (24), e1690 (open access)

5.        Yamanashi, K., Minamizaki, K. and Fujita, Y.  (2015) Identification of the chlE gene encoding oxygen-independent Mg-protoporphyrin IX monomethyl ester cyclase in cyanobacteria. Biochem. Biophys. Res. Commun., 463, 1328-1333; doi:10.1016/j.bbrc.2015.06.124

6.        Fujita, Y., Tsujimoto, R. and Aoki, R. (2015) Evolutionary aspects and regulation of tetrapyrrole biosynthesis in cyanobacteria under aerobic and anaerobic environments. Life, 5, 1172-1203; doi:103390/life5021172 (open access)

7.        Tsukatani, Y., Harada, J., Nomata, J., Yamamoto, H., Fujita, Y., Mizoguchi, T. and Tamiaki, H. (2015) Rhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthetic pathways. Scientific Reports 5, 9741. doi: 10.1038/srep-9741

8.        Hiraide, Y., Oshima, K., Fujisawa, T., Uesaka, K., Hirose, Y., Tsujimoto, R., Yamamoto, H., Okamoto, S., Nakamura, Y., Terauchi, K., Omata, T., Ihara, K., Hattori, M. and Fujita, Y. (2015) Loss of cytochrome cM stimulates cyanobacterial heterotrophic growth in the dark. Plant Cell Physiol., 56, 340-345.iPlant and Cell Physiology‚Ìweb page‚ÉResearch Highlights‚Æ‚µ‚ďЉ‚ê‚Ü‚µ‚½Bj

 

9.        Jiro Nomata, Toru Kondo, Tadashi Mizoguchi, Hitoshi Tamiaki, Shigeru Itoh and Fujita, Y. (2014) Dark-operative protochlorophyllide oxidoreductase generates substrate radicals by iron-sufphur cluster in bacteriochlorophyll biosynthesis. Scientific Reports, 4, 5455. doi:10.1038/srep05455

10.     Ryoma Tsujimoto, Narumi Kamiya, and Fujita, Y. (2014) Transcriptional regulators ChlR and CnfR are essential for diazotrophic growth of nonheterocystous cyanobacteria. Proc. Natl. Acad. Sci. USA 111, 6762-6767.

11.     Yamamoto, H., Kato, M., Yamanashi, K. and Fujita, Y. (2014) Reconstitution of a sequential reaction of two nitrogenase-like enzymes in the bacteriochlorophyll biosynthetic pathway of Rhodobacter capsulatus. Biochem. Biophys. Res. Commun. 448, 200-205.

12.     Aoki, R., Hiraide, Y., Yamakawa, H. and Fujita, Y. (2014) A novel goxygen-inducedh greening process in a cyanobacterial mutant lacking the transcriptional activator ChlR involved in low-oxygen adaptation of tetrapyrrole biosynthesis. J. Biol. Chem. 289, 1841-1851.

 

13.     Nomata, J., Kondo, T., Itoh, S. and Fujita, Y. (2013) Nicotinamide is a specific inhibitor of dark-operative protochlorophyllide oxidoreductase, a nitrogenase-like enzyme, from Rhodobacter capsulatus. FEBS Lett. 587, 3142-3147.

14.     Tsukatani, Y., Yamamoto, H., Mizoguchi, T., Fujita, Y. and Tamiaki, H. (2013) Completion of biosynthetic pathway for bacteriochlorophyll g in Heliobacterium modesticaldum: The C8-ethylidene group formation. Biochim. Biophys. Acta, 1827, 1200-1204.

15.     Tsukatani, Y., Yamamoto, H., Harada, J., Yoshitomi, T., Nomata, J., Kasahara, M., Mizoguchi, T., Fujita, Y. and Tamiaki, H. (2013) An unexpectedly branched biosynthetic pathway for bacteriochlorophyll b capable of absorbing near-infrared light. Sci. Rep. 3, 1217; DOI:10.1038/srep01217i—§–½ŠÙ‘åŠw‚ÌHP‚É“à—e‚ªÐ‰î‚³‚ê‚Ü‚µ‚½Bj

 

16.   Â–Ø—¢“ށA“¡“c—Sˆê (2012) ƒ‰ƒ“‘”‚̃eƒgƒ‰ƒsƒ[ƒ‹¶‡¬Œn‚ÌŒ™‹CŠÂ‹«“K‰ž‚Ɛi‰»“IŽ‹“_@Œõ‡¬Œ¤‹†@22, 87-97

17.   Aoki, R., Takeda, T., Omata, T., Ihara, K. and Fujita, Y. (2012) MarR-type transcriptional regulator ChlR activates expression of tetrapyrrole biosynthesis genes in response to low-oxygen conditions in cyanobacteria. J. Biol. Chem. 235, 13500-13507.

18.   Lee, C. G., Watanabe, T., Fujita, Y., Asakawa, S. and Kimura, M. (2012) Heterotrophic growth of cyanobacteria and phage-mediated loop in soil: Examination by stable isotope probing (SIP) method. Soil Sci. Plant Nutr. 58, 161-168.

 

19.   Yamamoto, H., Kurumiya, S., Ohashi, R. and Fujita, Y. (2011) 
Functional evaluation of a nitrogenase-like protochlorophyllide reductase encoded by the chloroplast DNA of Physcomitrella patens in the cyanobacterium Leptolyngbya boryana. Plant Cell Physiol. 52, 1983-1993.

20.   Aoki, R., Goto, T. and Fujita, Y. (2011) 
A heme oxygenase isoform is essential for aerobic growth in the cyanobacterium Synechocystis sp. PCC 6803: Modes of differential operation of two isoforms/enzymes to adapt low oxygen environments in cyanobacteria. Plant Cell Physiol. 52, 1744-1756.

21.   “¡“c—Sˆê (2011) ˆÃŠì“®Œ^ƒvƒƒgƒNƒƒƒtƒBƒŠƒhŠÒŒ³y‘f`ƒjƒgƒƒQƒi[ƒ[‚Ƃ̍\‘¢“I—ÞŽ—«‚©‚猩‚éy‘f‚Ì‘½—l«‚Ɛi‰»`@¶‰»Šw@83, 642-647.

22.   Takano, Y., Yonezawa, Y., Fujita, Y., Kurisu, G. and Nakamura, H. (2011) 
Electronic structures of a [4Fe-4S] cluster, [Fe4S4 (SCH3)3(CH3COO)], in dark-operative protochlorophyllide reductase (DPOR). Chem. Phys. Lett. 503, 296-300.

23.   “¡“c—SˆêAŒI²Œ¹Žk (2011) ƒNƒƒƒtƒBƒ‹‚ð—΂ɂ·‚é‚Q‚‚̊Ҍ³y‘fFŒõˆË‘¶Œ^y‘f‚ƈÏŠì“®Œ^y‘f‚Ì”½‰ž‹@\‚Ɛi‰»“IlŽ@@¶•¨•¨—@51, 66-71

24.   Kondo, T., Nomata, J., Fujita, Y. and Itoh, S. (2011) 
EPR study of 1Asp-3Cys ligated 4Fe-4S iron-sulfur cluster in NB-protein (BchN-BchB) of a dark-operative protochlorophyllide reductase complex. FEBS Lett. 585, 214-218.

 

25.   Reinbothe, C., El Bakkouri, M., Buhr, F., Muraki, N., Nomata, J., Kurisu, G., Fujita, Y. and Reinbothe, S. (2010) Chlorophyll biosynthesis: spotlight on protochlorophyllide reduction. Trends Plant Sci. 15, 614-624.i“¯Ž‚Ì11ŒŽ†‚Ì•\Ž†‚ðü‚è‚Ü‚µ‚½Ij

26.   ¬–“’B’jA“¡“c—SˆêA‘O“c^ˆê (2010) Œõ‡¬”÷¶•¨‚ÍŽ‘Œ¹EƒGƒlƒ‹ƒM[•ª–ì‚Ől—ނɍvŒ£‚Å‚«‚é‚©H`¶ŽY«‚ð‹K’è‚·‚鏔—vˆö‚Ì•ªÍ`@Œõ‡¬Œ¤‹†@20, 65-71.

27.   Imamura, S., Terashita, M., Ohnuma, M., Maruyama, S., Minoda, A., Weber, A. P. M., Inouye, T., Sekine, Y., Fujita, Y., Omata, T. and Tanaka, K. (2010) 
Nitrate assimilatory genes and their transcriptional regulation in a unicellular red alga Cyanidioschyzon merolae: Genetic evidence for nitrite reduction by a sulfite reductase-like enzyme. Plant Cell Physiol. 51, 707-717.

28.   Muraki, N., Nomata, J.,Ebata, K., Mizoguchi, T., Shibata, T., Tamiaki, H., Kurisu, G. and Fujita, Y. (2010) X-ray crystal structure of the light-independent protochlorophyllide reductase. Nature 465: 110-114

29.   Goto, T., Aoki, R., Minamizaki, K., and Fujita, Y. (2010) Functional differentiation of two analogous coproporphyrinogen III oxidases for heme and chlorophyll biosynthesis pathways in the cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol. 51: 650-663

 

30.   Yamamoto, H., Kurumiya, S., Ohashi, R and Fujita, Y. (2009) Oxygen sensitivity of a nitrogenase-like protochlorophyllide reductase from the cyanobacterium Leptolyngbya boryana. Plant Cell Physiol. 50, 1663-1673.

31.   “¡“c—SˆêA–ì–’ŽŸ˜Y (2009) Œõ‡¬×‹ÛRhodobacter capsulatus‚É‚¨‚¯‚é‘å—Ê”­Œ»‚ÆŒ`Ž¿“]Š·uŒõ‡¬Œ¤‹†–@v’ቷ‰ÈŠw67ŠªApp. 648-652A–kŠC“¹‘åŠw’ቷ‰ÈŠwŒ¤‹†Š

32.   “¡“c—SˆêA–ì–’ŽŸ˜Y (2009) Œ™‹CðŒ‰º‚ł̃^ƒ“ƒpƒNŽ¿¸»uŒõ‡¬Œ¤‹†–@v’ቷ‰ÈŠw67ŠªApp. 423-427A–kŠC“¹‘åŠw’ቷ‰ÈŠwŒ¤‹†Š

33.   Masuda, S., Ikeda, R., Masuda, T., Hashimoto, H., Tsuchiya, T., Kojima, H., Nomata, J., Fujita, Y., Mimuro, M., Ohta, H. and Takamiya, K. (2009) Prolamellar bodies formed by cyanobacterial protochlorophyllide oxidoreductase in Arabidopsis. Plant J. 58: 952-960.

34.   Masuda, T. and Fujita, Y. (2008)  Regulation and evolution of chlorophyll metabolism.  Photochem. Photobiol. Sci. 7, 1131-1149

35.   Yamamoto, H., Nomata, J., and Fujita, Y. (2008) Functional expression of nitrogenase-like protochlorophyllide reductase from Rhodobacter capsulatus in Escherichia coli. Photochem. Photobiol. Sci. 7, 1238-1242

36.   Nomata, J., Ogawa, T., Kitashima, M., Inoue, K., and Fujita, Y. (2008) NB-protein(BchN-BchB) of dark-operative protochlorophyllide reductase is the catalytic component containing oxygen-tolerant Fe-S clusters. FEBS Lett. 582, 1346-1350

37.   Minamizaki, K., Mizoguchi, T., Goto, T., Tamiaki, H., and Fujita Y. (2008) Identification of two homologous genes, chlAI and chlAII, that are differentially involved in isocyclic ring formation of chlorophyll a in the cyanobacterium Synechocystis sp. PCC 6803. J. Biol. Chem. 283, 2684-2692

38.   Nomata, J., Kitashima, M., Inoue, K. and Fujita, Y. (2006) Nitrogenase Fe protein-like Fe-S cluster is conserved in L-protein (BchL) of dark-operative protochlorophyllide reductase from Rhodobacter capsulatus. FEBS Lett. 580, 6151-6154

39.   Yamazaki, S., Nomata, J., and Fujita, Y. (2006) Differential operation of dual protochlorophyllide reductases for chlorophyll biosynthesis in response to environmental oxygen levels in the cyanobacterium Leptolyngbya boryana. Plant Physiol.142: 911-922

40.   Nomata, J., Mioguchi, T., Tamiaki, H., and Fujita, Y. (2006) A second nitrogenase-like enzyme for bacteriochlorophyll biosynthesis: Reconstitution of chlorophyllide a reductase with purified X-protein (BchX) and YZ-protein (BchY-BchZ) from Rhodobacter capsulatus. J. Biol. Chem. 281, 15021-15028. (JBC Paper of the Week‚É‘I‚΂ê‚Ü‚µ‚½)

41.   Nomata, J., Swem, L. R., Bauer, C. E. and Fujita, Y. (2005) Overexpression and characterization of dark-operative protochlorophyllide reductase from Rhodobacter capsulatus. Biochim. Biophys. Acta 1708, 229-237.

42.   Nomata, J., Inoue, K., Kitashima, M., Swem, L., Bauer, C. E. and Fujita, Y. (2004) Characterization of a nitrogenase-like enzyme catalyzing protochlorophyllide reduction from Rhodobacter capsulatus. In Photosynthesis: Fundamental Aspects to Global Perspectives, Edited by van der Est, A., Bruce, D., Allen Press, vol. 2, pp. 892-893.

43.   Terauchi, K., Katayama, M., Fujita, Y., Kondo, T. (2004) Circadian rhythm of the facultative cyanobacterium Plectonema boryanum. In Photosynthesis: Fundamental Aspects to Global Perspectives, Edited by van der Est, A., Bruce, D., Allen Press, vol. 2, pp. 729-730.

44.   “¡“c—Sˆê(2003) Œõ‡¬Ž«“T@“ú–{Œõ‡¬Œ¤‹†‰ï•Ò@Šw‰ïo”ŃZƒ“ƒ^[@iuƒNƒƒƒtƒBƒ‹ a ‚̐¶‡¬v‘¼‚V€–ځj

45.   Fujita, Y. and Bauer, C. E. (2003) The light-independent protochlorophyllide reductase: A nitrogenase-like enzyme catalyzing a key reaction for greening in the dark. In Porphyrin Handbook Vol. 13, edited by Kadish, K. M., Academic Press, pp.109-156.

46.   Kada, S., Koike, H., Satoh, K., Hase, T. and Fujita, Y. (2003) Arrest of chlorophyll synthesis and differential decrease of photosystems I and II in a cyanobacterial mutant lacking light-independent protochlorophyllide reductase. Plant Mol. Biol.  51, 225-235.

47.   Fujita, Y. (2002) Chlorophylls. In Encyclopedia of Life Science, Vol. 4, pp. 324-335. Nature Publishing Group, Macmillan Reference Limited, London.

48.   Inagaki, J., Fujita, Y., Hase, T. and Yamamoto, K. (2000) Protein translocation within chloroplast is similar in Euglena and higher plants. Biochem. Biophys. Res. Commun277, 436-442.

49.   Kimata-Ariga, Y., Matsumura, T., Kada, S., Fujimoto, S., Fujita, Y., Endo, T., Mano, J., Sato, F. and Hase, T. (2000) Differential electron flow around photosystem I by two C4-photosynthetic-cell-specific ferredoxins. EMBO J. 19, 5041-5050.

50.   Fujita, Y. and Bauer, C. E. (2000) Reconstitution of light-independent protochlorophyllide reductase from purified BchL and BchN-BchB subunits: In vitro confirmation of nitrogenase-like features of a bacteriochlorophyll biosynthesis enzyme. J. Biol. Chem. 275, 23583-23588.

51.   Okuhara, H., Matsumura, T., Fujita, Y. and Hase, T. (1999) Cloning and inactivation of genes encoding ferredoxin- and NADH-dependent glutamate synthases in the cyanobacterium Plectonema boryanum. Imbalances in nitrogen and carbon assimilations caused by deficiency of the ferredoxin-dependent enzyme. Plant Physiol. 120, 33-41.

52.   Okuhara, H., Matsumura, T., Fujita, Y. and Hase, T. (1998) Fd-GOGAT is necessary for the interaction of photosynthetic C- and N-assimilation in cyanobacterium. In Photosynthesis: Mechanism and Effects. Edited by Garab, G. Vol. V, pp. 3603-3606. Kluwer Academic Publishers, Dordrecht.

53.   Fujita, Y., Takagi, H. and Hase, T. (1998) Cloning of the gene encoding a protochlorophyllide reductase: the physiological significance of the co-existence of light-dependent and -independent protochlorophyllide reduction systems in the cyanobacterium Plectonema boryanum. Plant Cell Physiol. 39, 177-185.

54.   “¡“c—Sˆê(1996) ƒ^ƒ“ƒpƒNŽ¿ŽÀŒ±ƒm[ƒgiãj’Šo‚Æ•ª—£A—r“yŽÐA‘æ‚RÍ ×–E‚Ì”jÓ‚Æ•ª‰æi‚TjF59-62

55.   “¡“c—Sˆê(1996) ˆâ“`Žq‚ðƒNƒ[ƒjƒ“ƒO‚·‚éŽÀŒ±@VE¶•¨‰»ŠwŽÀŒ±‚Ì‚Ä‚Ñ‚«A’·’JrŽ¡•ÒA‰»Šw“¯l@‘æ‚QÍF19-63

56.   Fujita, Y. (1996) Protochlorophyllide reduction: a key step in the greening of plants. Plant Cell Physiol. 37, 411-421.

57.   Fujita, Y., Takagi, H. and Hase, T. (1996) Identification of the chlB gene and the gene product essential for the light-independent chlorophyll biosynthesis in the cyanobacterium Plectonema boryanum. Plant Cell Physiol. 37, 313-323.

58.   Suzuki, I., Kikuchi, H., Nakanishi, S., Fujita, Y., Sugiyama, T. and Omata, T. (1995) A novel nitrite reductase gene from the cyanobacterium Plectonema boryanum. J. Bacteriol177, 6137-6143.

59.   Fujita, Y., Takagi, H. and Hase, T. (1995) Genetic analysis of protochlorophyllide reduction in the cyanobacterium Plectonema boryanum. In Photosynthesis: from Light to Biosphere. Edited by Mathis, P. Vol. III, pp. 961-964. Kluwer Academic Publishers, Dordrecht.

60.   Matsumura, T., Fujita, Y. and Hase, T. (1995) Non-photosynthetic type ferredoxin from maize retards photosynthetic growth of the cyanobacterium Plectonema boryanum. In Photosynthesis: from Light to Biosphere. Edited by Mathis, P. Vol. II, pp. 709-712. Kluwer Academic Publishers, Dordrecht.

61.   Matsubara, H., Oh-oka, H., Takahashi, Y. and Fujita, Y. (1995) Three iron-sulfur proteins encoded by three ORFs in chloroplasts and cyanobacteria. Photosynth. Res. 46, 107-115.

62.   “¡“c—Sˆê(1994) Œõ”ñˆË‘¶«‚̃NƒƒƒtƒBƒ‹‡¬Œn‚ƃjƒgƒƒQƒi[ƒ[Œn@‰»Šw‚Ɛ¶•¨@‘æ32ŠªA‘æ5†F337-342.

63.   Fujita, Y., Matsumoto, H., Takahashi, Y. and Matsubara, H. (1993) Identification of a nifDK-like gene (ORF467) involved in the biosynthesis of chlorophyll in the cyanobacterium Plectonema boryanum. Plant Cell Physiol. 34, 305-314.

64.   Fujita, Y., Takahashi, Y., Oh-oka, H., Saeki, K., Fukuyama, K. and Matsubara, H. (1992) Evolutionary aspects of iron-sulfur proteins in photosynthetic apparatus. In Research in Photosynthesis. Edited by Murata, N. Vol. III, pp. 491-498. Kluwer Academic Publishers, Dordrecht.

65.   Fujita, Y., Takahashi, Y., Shonai, F., Matsumoto, H. and Matsubara, H. (1992) The light-independent reduction of protochlorophyllide in the cyanobacterium Plectonema boryanum. In Research in Photosynthesis. Edited by Murata, N. Vol. III, pp. 67-70. Kluwer Academic Publishers, Dordrecht.

66.   Yamada, K., Matsuda, M., Fujita, Y., Matsubara, H. and Sugai, M. (1992) A frxC homolog exists in the chloroplast DNAs from various pteridophytes and gymnosperms. Plant Cell Physiol. 33,  325-327.

67.   Fujita, Y., Takahashi, Y., Chuganji, M. and Matsubara, H. (1992) The nifH-like (frxC) gene is involved in the biosynthesis of chlorophyll in the filamentous cyanobacterium Plectonema boryanum. Plant Cell Physiol. 33, 81-92. (“ú–{A•¨¶—Šw‰ï˜_•¶Ü)

68.   Fujita, Y., Takahashi, Y., Shonai, F., Ogura, Y. and Matsubara, H. (1991) Cloning, nucleotide sequences and differential expression of the nifH and nifH-like (frxC) genes from the filamentous nitrogen-fixing cyanobacterium Plectonema boryanum. Plant Cell Physiol. 32, 1093-1106.

69.   Takahashi, Y., Shonai, F., Fujita, Y., Kohchi, T., Ohyama, K. and Matsubara, H. (1991) Structure of a co-transcribed gene cluster, ndh1-frxB-ndh6-ndh4L, cloned from the filamentous cyanobacterium Plectonema boryanum. Plant Cell Physiol. 32, 969-981.

70.   Takahashi, Y., Mitsui, A., Fujita, Y. and Matsubara, H. (1991) Roles of ATP and NADPH in formation of the Fe-S cluster of spinach ferredoxin. Plant Physiol. 95, 104-110.

71.   Fujita, Y., Takahashi, Y., Kohchi, T., Ozeki, H., Ohyama, K. and Matsubara, H. (1989) Identification of a novel nifH-like (frxC) protein in chloroplasts of the liverwort Marchantia polymorpha. Plant Mol. Biol. 13, 551-561.

 

 

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The 5th International Conference on MEXT Project of Integrated Research on Chemical Synthesis gChemical Science for Future Societieshi–¼ŒÃ‰®‘åŠwj

Yuichi Fujita, Nitrogenase-like reductases in bacteriochlorophyll biosynthesis: Reaction mechanism and plasticity, January 29–30, 2016, Nagoya University, Japan

 

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Yuichi Fujita, Reaction mechanism of a nitrogenase-like protochlorophyllide reductase. 19th International Congress on Nitrogen Fixation, October 4–9, 2015, Pacific Grove, CA, USA

 

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Yuto Hiraide, Kazuma Uesaka, Kunio Ihara and Yuichi Fujita, Loss of the cytM gene encoding cytochrome cM stimulates dark heterotrophic growth of cyanobacteria. 15th International Symposium on Phototrophic Prokaryotes, August 2–6, Tübingen, Germany

 

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Yuichi Fujita, CnfR is a master transcriptional activator essential for nitrogen fixation in nonheterocystous cyanobacteria. 3rd Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation, October 28-30, 2-14, 2014, Chengdu, China

 

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Yuichi Fujita, Chlorophyll biosynthesis under low oxygen environments. Institute for Protein Research Seminar, Regulation of Environmental Adaptation of Photosynthesis: An Attractive Theme for Structural Life Sciences, October 24, 2014, Osaka University, Japan

 

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Yuichi Fujita and Ryoma Tsujimoto, Two transcriptional regulators, ChlR and PatB, are essential for diazotrophic growth in the non-heterocystous cyanobacterium Leptolyngbya boryana. October 14-18, 2013, Miyazaki, Japan

 

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Yuichi Fujita, Nitrogenase-like enzymes in bacteriochlorophyll biosynthesis. October 11-12, 2013, Nagoya University, Japan (Invited speaker and Organizer)

 

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Yuichi Fujita, Mina Kato and Haruki Yamamoto, Reconstitution of sequential reaction from protochlorophyllide to 3-vinyl bacteriochlorophyllide a by two nitrogenase-like enzymes. September 11-15, 2013, HuaZong Agricultural University, Wuhan, China

 

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Yuichi Fujita, Rina Aoki and Ryoma Tsujimoto, Chlorophyll biosynthesis under anaerobic conditions. 2nd International Symposium on Biosynthesis of Tetrapyrroles. November 30-December 2, 2012, Ritsumeikan University, Shiga, Japan

 

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Yuichi Fujita, Progress on crystallographic and biochemical analysis of DPOR, a nitrogenase-like enzyme. Gordon Research Conference, Iron-sulfur enzymes, June 10-15, 2012, Mount Holyoke College, South Hadley, MA, USA

 

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Yuichi Fujita, Toward understanding of reaction mechanism of dark-operative protochlorophyllide reductase. International Conference on Tetrapyrrole Photoreceptors of Photosynthetic organisms, July 24-28, 2011, Berlin, Germany

 

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Yuichi Fujita, Structure-based functional analysis on dark-operative protochlorophyllide reductase. Gordon Research Conference, Photosynthesis, June 12-17, 2011, Davidson College, Davidson, NC, USA

 

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Yuichi Fujita, Norifumi Muraki, Jiro Nomata and Genji Kurisu, A common architecture between protochlorophyllide reductase and nitrogenase. 1st Asian Symposium on Plant-Microbe Symbiosis and Nitrogen Fixation, September 20-24, 2010, Miyazaki, Japan

 

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Yuichi Fujita, Norifumi Muraki, Jiro Nomata and Genji Kurisu, Structure of the light-independent protochlorophyllide reductase reveals architecture common to nitrogenase. 9th European Conference on Nitrogen Fixation, September 6-10, 2010, Geneva, Switzerland

 

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Yuichi Fujita, Genji Kurisu, Norifumi Muraki and Jiro Nomata, Structural aspects of dark-operative protochlorophyllide reductase with nitrogenase-like features. 6th International Conference on Porphyrins and Phthalocyanines, July 4-9, 2010, Santa Ana Pueblo, NM, USA

 

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Yuichi Fujita, Structural basis of nitrogenase-like dark-operative protochlorophyllide reductase catalyzing porphyrin reduction. 16th International Conference on Nitrogen Fixation, June 14-19, 2009, Big Sky, Montana, USA

 

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Yuichi Fujita, Nitrogenase homologues in chlorophyll biosynthesis. Gordon Research Conference on Iron-Sulfur Enzymes, June 7-13, 2008, Colby Sawyer College, New London, NH, USA

 

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Yuichi Fujita, Final two stages of chlorophyllide a formation in chlorophyll biosynthesis: E-ring formation and D-ring reduction. 7th International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms, December 9-14, 2007, Kyoto, Japan

 

 

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