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1.
New! Terauchi, K., Sobue, R., Furutani, Y., Aoki, R., and Fujita, Y. (2016) Isolation of cyanobacterial
mutants exhibiting growth defects under microoxic
conditions by transposon mutangenesis of Synechocystis sp. PCC 6803. J. Gen. Appli. Microbiol, in press.
2.
New! Tsujimoto, R., Kamiya, N., and
Fujita, Y. (2016) Identification of
a cis-acting element in nitrogen
fixation genes recognized by CnfR in the nonheterocystous
nitrogen-fixing cyanobacterium Leptolyngbya
boryana. Mol. Microbiol.,
101, 411-424, doi:10.1111/mmi.13402
3.
Nomata,
J., Terauchi, K., and Fujita, Y.
(2016) Stoichiometry of ATP hydrolysis and chlorophyllide
formation of dark-operative protochlorophyllide oxidoreductase from Rhodobacter capsulatus. Biochem. Biophys. Res. Commun., 470,
704-709, doi:10.1016/j.bbrc.2016.01.070
4.
Tsujimoto,
R., Kotani, H., Nonaka, A.,
Miyahara, Y, Hiraide, Y. and Fujita, Y. (2015) Transformation of the
cyanobacterium Leptolyngbya boryana
by electroporation. Bio-Protocol, 5
(24), e1690 (open access)
5.
Yamanashi,
K., Minamizaki, K. and Fujita, Y. (2015) Identification of the chlE gene
encoding oxygen-independent Mg-protoporphyrin IX monomethyl ester cyclase in
cyanobacteria. Biochem. Biophys. Res. Commun., 463, 1328-1333; doi:10.1016/j.bbrc.2015.06.124
6.
Fujita,
Y., Tsujimoto, R. and Aoki, R. (2015) Evolutionary aspects and regulation of tetrapyrrole biosynthesis in cyanobacteria under aerobic
and anaerobic environments. Life, 5, 1172-1203; doi:103390/life5021172
(open access)
7.
Tsukatani,
Y., Harada, J., Nomata, J., Yamamoto, H., Fujita, Y., Mizoguchi, T. and Tamiaki, H. (2015) Rhodobacter sphaeroides mutants overexpressing chlorophyllide
a oxidoreductase
of Blastochloris viridis
elucidate functions of enzymes in late bacteriochlorophyll
biosynthetic pathways. Scientific Reports
5, 9741. doi:
10.1038/srep-9741
8.
Hiraide, Y., Oshima, K., Fujisawa, T., Uesaka,
K., Hirose, Y., Tsujimoto, R., Yamamoto, H., Okamoto, S., Nakamura, Y.,
Terauchi, K., Omata, T., Ihara, K., Hattori, M. and Fujita, Y. (2015) Loss of
cytochrome cM
stimulates cyanobacterial heterotrophic growth in the dark. Plant Cell Physiol., 56, 340-345.iPlant and Cell Physiology‚Ìweb page‚ÉResearch Highlights‚Æ‚µ‚ÄЉ‚ê‚Ü‚µ‚½Bj
9.
Jiro Nomata, Toru Kondo, Tadashi
Mizoguchi, Hitoshi Tamiaki, Shigeru Itoh and Fujita, Y. (2014) Dark-operative protochlorophyllide oxidoreductase
generates substrate radicals by iron-sufphur cluster
in bacteriochlorophyll biosynthesis. Scientific
Reports, 4, 5455. doi:10.1038/srep05455
10.
Ryoma Tsujimoto, Narumi Kamiya, and Fujita, Y. (2014) Transcriptional regulators ChlR and CnfR are essential for diazotrophic
growth of nonheterocystous cyanobacteria. Proc.
Natl. Acad. Sci. USA 111, 6762-6767.
11.
Yamamoto, H., Kato,
M., Yamanashi, K. and Fujita, Y. (2014) Reconstitution of a sequential reaction
of two nitrogenase-like enzymes in the bacteriochlorophyll
biosynthetic pathway of Rhodobacter capsulatus. Biochem. Biophys. Res. Commun. 448, 200-205.
12.
Aoki, R., Hiraide, Y., Yamakawa, H. and
Fujita, Y. (2014) A novel goxygen-inducedh greening process in a cyanobacterial
mutant lacking the transcriptional activator ChlR
involved in low-oxygen adaptation of tetrapyrrole
biosynthesis. J. Biol. Chem. 289, 1841-1851.
13.
Nomata, J.,
Kondo, T., Itoh, S. and Fujita, Y. (2013) Nicotinamide is a specific inhibitor of dark-operative protochlorophyllide oxidoreductase,
a nitrogenase-like enzyme, from Rhodobacter capsulatus. FEBS Lett.
587, 3142-3147.
14.
Tsukatani, Y.,
Yamamoto, H., Mizoguchi, T., Fujita, Y. and Tamiaki,
H. (2013) Completion of biosynthetic pathway for bacteriochlorophyll
g in Heliobacterium modesticaldum:
The C8-ethylidene group formation. Biochim. Biophys. Acta, 1827, 1200-1204.
15.
Tsukatani, Y.,
Yamamoto, H., Harada, J., Yoshitomi, T., Nomata, J., Kasahara,
M., Mizoguchi, T., Fujita, Y. and Tamiaki, H.
(2013) An unexpectedly branched biosynthetic pathway for bacteriochlorophyll
b capable of absorbing near-infrared
light. Sci. Rep. 3, 1217; DOI:10.1038/srep01217i—§–½ŠÙ‘åŠw‚ÌHP‚É“à—e‚ªÐ‰î‚³‚ê‚Ü‚µ‚½Bj
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17.
Aoki, R., Takeda, T., Omata, T., Ihara, K. and Fujita, Y.
(2012) MarR-type transcriptional regulator ChlR activates expression of tetrapyrrole
biosynthesis genes in response to low-oxygen conditions in cyanobacteria. J.
Biol. Chem. 235, 13500-13507.
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S. and Kimura, M. (2012) Heterotrophic growth of cyanobacteria and
phage-mediated loop in soil: Examination by stable isotope probing (SIP)
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19.
Yamamoto, H., Kurumiya, S., Ohashi, R. and Fujita, Y. (2011)
Functional
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reductase encoded by the chloroplast DNA of Physcomitrella patens in the cyanobacterium Leptolyngbya boryana. Plant
Cell Physiol. 52, 1983-1993.
20.
Aoki, R., Goto, T. and Fujita, Y.
(2011)
A heme oxygenase
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differential operation of two isoforms/enzymes to adapt low oxygen environments
in cyanobacteria. Plant Cell Physiol. 52, 1744-1756.
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EPR study of 1Asp-3Cys ligated 4Fe-4S iron-sulfur cluster in
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585, 214-218.
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M., Buhr, F., Muraki, N., Nomata, J., Kurisu, G., Fujita,
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Muraki, N., Nomata, J.,Ebata, K.,
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and Fujita, Y. (2010) X-ray crystal structure of the light-independent protochlorophyllide reductase. Nature 465: 110-114
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Inoue, K., and Fujita, Y. (2008) NB-protein(BchN-BchB)
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The 5th International Conference on MEXT Project of
Integrated Research on Chemical Synthesis gChemical Science for Future
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Yuichi Fujita, Nitrogenase-like reductases in bacteriochlorophyll
biosynthesis: Reaction mechanism and plasticity, January 29–30, 2016,
Nagoya University, Japan
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Yuichi Fujita, Reaction mechanism
of a nitrogenase-like protochlorophyllide reductase.
19th International Congress on Nitrogen Fixation, October 4–9,
2015, Pacific Grove, CA, USA
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Yuto Hiraide, Kazuma
Uesaka, Kunio Ihara and Yuichi
Fujita, Loss of the cytM gene encoding cytochrome
cM stimulates dark heterotrophic growth of
cyanobacteria. 15th International Symposium on Phototrophic
Prokaryotes, August 2–6, Tübingen, Germany
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Yuichi Fujita, CnfR is a master
transcriptional activator essential for nitrogen fixation in nonheterocystous cyanobacteria. 3rd Asian
Conference on Plant-Microbe Symbiosis and Nitrogen Fixation, October 28-30,
2-14, 2014, Chengdu, China
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Yuichi Fujita, Chlorophyll
biosynthesis under low oxygen environments. Institute for Protein Research
Seminar, Regulation of Environmental Adaptation of Photosynthesis: An
Attractive Theme for Structural Life Sciences, October 24, 2014, Osaka
University, Japan
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Yuichi Fujita and Ryoma Tsujimoto, Two
transcriptional regulators, ChlR and PatB, are essential for diazotrophic
growth in the non-heterocystous cyanobacterium
Leptolyngbya boryana. October 14-18, 2013, Miyazaki, Japan
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Yuichi Fujita, Nitrogenase-like enzymes in bacteriochlorophyll
biosynthesis. October 11-12, 2013, Nagoya University, Japan (Invited speaker
and Organizer)
Œõ‡¬¶•¨‚̃eƒgƒ‰ƒsƒ[ƒ‹ŒõŽó—e‘̂Ɋւ·‚é‘Û‰ï‹c iICTPPO2013ji’†‘A•Š¿j
Yuichi Fujita, Mina Kato and Haruki
Yamamoto, Reconstitution of sequential reaction from protochlorophyllide
to 3-vinyl bacteriochlorophyllide a by two
nitrogenase-like enzymes. September 11-15, 2013, HuaZong
Agricultural University, Wuhan, China
‘æ2‰ñƒeƒgƒ‰ƒsƒ[ƒ‹¶‡¬‘ÛƒVƒ“ƒ|ƒWƒEƒ€i—§–½ŠÙ‘åŠwj
Yuichi Fujita, Rina Aoki and Ryoma
Tsujimoto, Chlorophyll biosynthesis under anaerobic conditions. 2nd
International Symposium on Biosynthesis of Tetrapyrroles.
November 30-December 2, 2012, Ritsumeikan University,
Shiga, Japan
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Yuichi Fujita, Progress on crystallographic
and biochemical analysis of DPOR, a nitrogenase-like enzyme. Gordon Research
Conference, Iron-sulfur enzymes, June 10-15, 2012, Mount Holyoke College, South
Hadley, MA, USA
Œõ‡¬¶•¨‚̃eƒgƒ‰ƒsƒ[ƒ‹ŒõŽó—e‘̂Ɋւ·‚é‘Û‰ï‹c iICTPPO2011jiƒhƒCƒcAƒxƒ‹ƒŠƒ“j
Yuichi Fujita, Toward
understanding of reaction mechanism of dark-operative protochlorophyllide
reductase. International Conference on Tetrapyrrole
Photoreceptors of Photosynthetic organisms, July 24-28, 2011, Berlin, Germany
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Yuichi Fujita,
Structure-based functional analysis on dark-operative protochlorophyllide
reductase. Gordon Research Conference, Photosynthesis, June 12-17, 2011,
Davidson College, Davidson, NC, USA
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Yuichi Fujita, Norifumi
Muraki, Jiro Nomata and Genji
Kurisu, A common architecture between protochlorophyllide
reductase and nitrogenase. 1st Asian Symposium on Plant-Microbe Symbiosis and
Nitrogen Fixation, September 20-24, 2010, Miyazaki, Japan
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Yuichi Fujita, Norifumi
Muraki, Jiro Nomata and Genji
Kurisu, Structure of the light-independent protochlorophyllide
reductase reveals architecture common to nitrogenase. 9th European Conference
on Nitrogen Fixation, September 6-10, 2010, Geneva, Switzerland
‘æ6‰ñƒ|ƒ‹ƒtƒBƒŠƒ“‚ƃtƒ^ƒƒVƒAƒjƒ“‚ÉŠÖ‚·‚é‘Û‰ï‹ci•Ä‘ƒjƒ…[ƒƒLƒVƒRBAƒTƒ“ƒ^ƒAƒiƒuƒGƒuƒj
Yuichi Fujita, Genji
Kurisu, Norifumi Muraki and Jiro
Nomata, Structural aspects of dark-operative protochlorophyllide
reductase with nitrogenase-like features. 6th International Conference on Porphyrins and Phthalocyanines,
July 4-9, 2010, Santa Ana Pueblo, NM, USA
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Yuichi Fujita, Structural
basis of nitrogenase-like dark-operative protochlorophyllide
reductase catalyzing porphyrin reduction. 16th
International Conference on Nitrogen Fixation, June 14-19, 2009, Big Sky,
Montana, USA
ƒS[ƒhƒ“‰ï‹ci“S—°‰©y‘fji•Ä‘ƒjƒ…[ƒnƒ“ƒvƒVƒƒ[BAƒjƒ…[ƒƒ“ƒhƒ“j
Yuichi Fujita, Nitrogenase homologues in chlorophyll biosynthesis.
Gordon Research Conference on Iron-Sulfur Enzymes, June 7-13, 2008, Colby
Sawyer College, New London, NH, USA
‘æ7‰ñŒõ‡¬¶•¨‚̃eƒgƒ‰ƒsƒ[ƒ‹ŒõŽó—e‘̂Ɋւ·‚é‘Û‰ï‹c (7th ICTPPO)i‹ž“sj
Yuichi Fujita, Final two stages of chlorophyllide
a formation in chlorophyll
biosynthesis: E-ring formation and D-ring reduction. 7th International
Conference on Tetrapyrrole Photoreceptors in Photosynthetic
Organisms, December 9-14, 2007, Kyoto, Japan