List of major publications related to current research projects during 2001- up to now

* Review articles

High Sensitive Quantitative Binding Assays Using a Nanoluciferase-Fused Probe for Analysis of ALG-2-Interacting Proteins.
Zhang W, Matsuo R, Takahara T, Shibata H, Maki M.
Methods Mol Biol. 2019;1929:501-516. doi: 10.1007/978-1-4939-9030-6_31.
PMID: 30710293

Cellular Ca²⁺-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-palindromic NFAT-Response Elements.
Zhang W, Takahara T, Achiha T, Shibata H, Maki M.
Methods Mol Biol. 2019;1929:95-109. doi: 10.1007/978-1-4939-9030-6_7.
PMID: 30710269

Adaptor functions of the Ca²⁺-binding protein ALG-2 in protein transport from the endoplasmic reticulum.
Shibata H.
Biosci Biotechnol Biochem. 2019 Jan;83(1):20-32. doi: 10.1080/09168451.2018.1525274. Epub 2018 Sep 27 Review
PMID:.30259798

Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements Facilitates Analysis of Biological Endpoint Effects of Cellular Ca2+ Mobilization.
Zhang W, Takahara T, Achiha T, Shibata H, Maki M.
Int J Mol Sci. 2018 Feb 18;19(2). pii: E605. doi: 10.3390/ijms19020605.
PMID: 29463029

A microtubule-associated protein MAP1B binds to and regulates localization of a calcium-binding protein ALG-2.
Takahara T, Arai Y, Kono Y, Shibata H, Maki M.
Biochem Biophys Res Commun. 2018 Mar 4;497(2):492-498. doi: 10.1016/j.bbrc.2018.02.048. Epub 2018 Feb 10.
PMID: 29432744

The calcium-binding protein ALG-2 regulates protein secretion and trafficking via interactions with MISSL and MAP1B proteins.
Takahara T, Inoue K, Arai Y, Kuwata K, Shibata H, Maki M.
J Biol Chem. 2017 Oct 13;292(41):17057-17072. doi: 10.1074/jbc.M117.800201. Epub 2017 Sep 1. PMID:28864773

Mutations in the vesicular trafficking protein annexin A11 are associated with amyotrophic lateral sclerosis.
Smith BN, Topp SD, Fallini C, Shibata H,,,,,,Maki M,,,,,,, Shaw CE2
Sci Transl Med. 2017 May 3;9(388). pii: eaad9157.
(国際共同研究）概要はこちら　⇒　ライフサイエンス 新着論文レビュー

The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein.
Kanadome T, Shibata H, Kuwata K, Takahara T, Maki M.
FEBS J. 2017 Jan;284(1):56-76. doi: 10.1111/febs.13949.PMID: 27813252
Free access

Multifaceted Roles of ALG-2 in Ca2+ -Regulated Membrane Trafficking.
Maki M, Takahara T, Shibata H.
Int J Mol Sci. 2016 Aug 26;17(9). pii: E1401. doi: 10.3390/ijms17091401. Review.
Free access

Tubby-like protein superfamily member PLSCR3 functions as a negative regulator of adipogenesis in mouse 3T3-L1 preadipocytes by suppressing induction of late differentiation stage transcription factors
Inokawa A, Inuzuka T, Takahara T, Shibata H, Maki M.
Biosci Rep. 2015 Dec 16;36(1). pii: e00287. doi: 10.1042/BSR20150215. PMID: 26677203
Free access

Structural Analysis of the Complex between Penta-EF-Hand ALG-2 Protein and Sec31A Peptide Reveals a Novel Target Recognition Mechanism of ALG-2.
Takahashi T, Kojima K, Zhang W, Sasaki K, Ito M, Suzuki H, Kawasaki M, Wakatsuki S, Takahara T, Shibata H, Maki M.
Int J Mol Sci. 2015 Feb 6;16(2):3677-99. doi: 10.3390/ijms16023677. PPMID: 25667979
Free access (at mdpi)

A New Role for Annexin A11 in the Early Secretory Pathway via Stabilizing Sec31A at the Endoplasmic Reticulum Exit Sites (ERES).
Shibata H, Kanadome T, Sugiura H, Yokoyama T, Yamamuro M, Moss SE, Maki M.
J Biol Chem. 2015 Feb 20; 2290(8):4981-93.PMID: 25540196

Involvement of calpain-7 in epidermal growth factor receptor degradation via the endosomal sorting pathway.
Maemoto Y, Ono Y, Kiso S, Shibata H, Takahara T, Sorimachi H, Maki M.
FEBS J. 2014 Aug;281(16):3642-55. doi: 10.1111/febs.12886. Epub 2014 Jul 8.
PMID: 24953135

Nuclear ALG-2 Protein Interacts with Ca2+ Homeostasis Endoplasmic Reticulum Protein (CHERP) Ca2+-dependently and Participates in Regulation of Alternative Splicing of Inositol Trisphosphate Receptor Type 1 (IP3R1) Pre-mRNA.
J Biol Chem. 2013 Nov 15;288(46):33361-75. doi: 10.1074/jbc.M113.497479. Epub 2013 Sep 27.

VPS37 Isoforms Differentially Modulate the Ternary Complex Formation of ALIX, ALG-2, and ESCRT-I.
Okumura M, Katsuyama AM, Shibata H, Maki M.
Biosci Biotechnol Biochem. 2013 Aug 23;77(8):1715-21. Epub 2013 Aug 7.
PMID: 23924735 Free access

Identification of phosphorylation sites in the C-terminal region of charged multivesicular body protein 1A (CHMP1A).
Maemoto Y, Shibata H, Maki M.
Biosci Biotechnol Biochem. 2013;77(6):1317-9. Epub 2013 Jun 7.
PMID: 23748770 Free access

Analysis of limited proteolytic activity of calpain-7 using non-physiological substrates in mammalian cells.
Maemoto Y, Kiso S, Shibata H, Maki M.
FEBS J. 2013 Jun;280(11):2594-607. doi: 10.1111/febs.12243. Epub 2013 Apr 8.
PMID: 23497113

ALG-2-interacting Tubby-like protein superfamily member PLSCR3 is secreted by exosomal pathway and taken up by recipient cultured cells.
Inuzuka T, Inokawa A, Chen C, Kizu K, Narita H, Shibata H, Maki M.
Biosci Rep. 2013 Jan 26. [Epub ahead of print] 23350699 Free access

Mammalian ESCRT-III-Related Protein IST1 Has a Distinctive Met-Pro Repeat Sequence That Is Essential for Interaction with ALG-2 in the Presence of Ca(2+).
Okumura M, Takahashi T, Shibata H, Maki M.Biosci Biotechnol Biochem. 2013 May 23;77(5):1049-54. Epub 2013 May 7

Prediction of a New Ligand-Binding Site for Type 2 Motif Based on the Crystal Structure of ALG-2 by Dry and Wet Approaches
Takahashi T, Suzuki H, Inuzuka T, Shibata H, Maki M. Int. J. Mol. Sci. 2012, 13, 7532-7549; doi:10.3390/ijms13067532　　Free access

Identification of the P-body component PATL1 as a novel ALG-2-interacting protein by in silico and Far-Western screening of proline-rich proteins.
ESCRT and calpain - old and new relationships.*
Evolutionary and physical linkage between calpains and penta-EF-hand Ca(2+) -binding proteins. *
Structure and function of ALG-2, a penta-EF-hand calcium-dependent adaptor protein .*
Hepatitis C virus hijacks P-body and stress granule components around lipid droplets.
Ariumi Y, Kuroki M, Kushima Y, Osugi K, Hijikata M, Maki M, Ikeda M, Kato N.
J Virol. 2011 Jul;85(14):6882-92. Epub 2011 May 4.

Calpain-7 binds to CHMP1B at its second α-helical region and forms a ternary complex with IST1.
The ESCRT system is required for hepatitis C virus production.
Ariumi Y, Kuroki M, Maki M, Ikeda M, Dansako H, Wakita T, Kato N.
PLoS One. 2011 Jan 11;6(1):e14517.

Autolytic activity of human calpain 7 is enhanced by ESCRT-III-relatedprotein IST1 through MIT-MIM interaction.
Osako Y, Maemoto Y Tanaka R, Suzuki H, Shibata H,  Maki M.
FEBS J 277 (21) (2010)4412-4426 November

The ALG-2 Binding Site in Sec31A Influences Retention Kinetics of Sec31A at the Endoplasmic Reticulum Exit Sites as Revealed by Live-Cell Time-Lapse Imaging
Shibata H, Inuzuka T, Yoshida H, Sugiura H, Wada I, and Maki M
Biosci. Biotech. Biochem.　 2010 Sep 23;74(9):1819-26.

Molecular basis for defect in Alix-binding by alternatively spliced isoform of ALG-2 (ALG-2^ΔGF122) and structural roles of F122 in target recognition
Inuzuka T, Suzuki H, Kawasaki M, Shibata M, Wakatsuki S, and Maki M,
BMC Structural Biology BMC Struct Biol. 2010 Aug 6;10:25 (page 1-14) Free access

Distinct functions of human MVB12A and MVB12B in the ESCRT-I dependent on their posttranslational modifications. Tsunematsu T, Yamauchi E, Shibata H, Maki M, Ohta T, Konishi H. Biochem Biophys Res Commun. 2010 Aug 20;399(2):232-7. Epub 2010 Jul 21. Erratum in: Biochem Biophys Res Commun. 2011 Mar 4;406(1):158-9.

Penta-EF-hand protein ALG-2 functions as a Ca2+-dependent adaptor that bridges Alix and TSG101.
Okumura M, Ichioka F, Kobayashi R, Suzuki H, Yoshida H, Shibata H, Maki M.
Biochem Biophys Res Commun. 2009 Aug 14;386(1):237-241.

The mechanism of Ca2+-dependent recognition of Alix by ALG-2: insights from X-ray crystal structures.
Suzuki H, Kawasaki M, Inuzuka T, Okumura M, Kakiuchi T, Shibata H, Wakatsuki S, Maki M.
Biochem Soc Trans. 2009 Feb;37(Pt 1):190-194. Review.

Crystalization and X-ray diffraction analysis of N-terminally truncated human ALG-2
Suzuki H, Kawasaki M, Kakiuchi T, Shibata H, Wakatsuki S, Maki M.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):974-977.

Structural basis for Ca2+ -dependent formation of ALG-2/Alix peptide complex: Ca2+/EF3-driven arginine switch mechanism.
Suzuki H, Kawasaki M, Inuzuka T, Okumura M, Kakiuchi T, Shibata H, Wakatsuki S, Maki M.
Structure. 2008 Oct 8;16(10):1562-1573.

Human calpain 7/PalBH associates with a subset of ESCRT-III-related proteins in its N-terminal region and partly localizes to endocytic membrane compartments.
Yorikawa C, Takaya E, Osako Y, Tanaka R, Terasawa Y, Hamakubo T, Mochizuki Y, Iwanari H, Kodama T, Maeda T, Hitomi K, Shibata H, Maki M., J Biochem. 2008 Jun;143(6):731-45

Brox, a novel farnesylated Bro1 domain-containing protein that associates with charged multivesicular body protein 4 (CHMP4) 
Fumitaka Ichioka¹, Ryota Kobayashi, Keiichi Katoh, Hideki Shibata and Masatoshi Maki
FEBS J. 2008 Feb;275(4):682-692.

Adaptor protein Ruk/CIN85 is associated with a subset of COPI coated membranes of the Golgi complex.
Havrylov S,Ichioka F, Powell K, Borthwick EB, Baranska J, Maki M, Buchman VL.
Traffic.9(5):798-812 (2008).

Identificationof alix-type and non-alix-type ALG-2-binding sites in human phospholipidscramblase 3: Differential binding to an alternatively spliced isoform andamino acid-substituted mutants.
Shibata H, Suzuki H, Kakiuchi T, Inuzuka T, Yoshida H, Mizuno T, Maki M.
J Biol Chem. 2008 Apr 11;283(15):9623-32.

The Penta-EF-hand Protein ALG-2 and Its Interacting Proteins.
Maki, M. and Shibata, H.
Calcium Binding Proteins 2, 4-10 (2007)

ALG-2 directly binds Sec31A and localizes at endoplasmic reticulum exit sites in a Ca2+-dependent manner.
Biochem Biophys Res Commun. 2007 Feb 16;353(3):756-763.
Shibata H, Suzuki H, Yoshida H, Maki M.
Biochem Biophys Res Commun. 2007 Feb 16;353(3):756-63. Epub 2006 Dec 22.

HD-PTP and Alix share some membrane-traffic related proteins that interact with their Bro1 domains or proline-rich regions.
Ichioka F, Takaya E, Suzuki H, Kajigaya S, Buchman VL, Shibata H, Maki M.
Arch Biochem Biophys. (2007) 457(2):142-9.

CHMP7, a novel ESCRT-III-related protein, associates with CHMP4b and functions in the endosomal sorting pathway.
Horii M, Shibata H, Kobayashi R, Katoh K, Yorikawa C, Yasuda J, Maki M.
Biochem J. (2006) 400, 23-32.

The penta-EF-hand protein ALG-2 directly interacts with the ESCRT-I componentTSG101 and Ca2+ 2+-dependently colocalizes to aberrant endosomes with dominant-negativeAAA ATPase SKD1/Vps4B.
Katoh K, Suzuki H, Terasawa Y, Mizuno T, Yasuda J, Shibata H, Maki M.
Biochem. J. (2005) 391(Pt 3):677-685

Reevaluation of the predicted gene structure of Dictyostelium cystatin A3 (cpiC) by the nucleotide sequence determination of its cDNA and its phylogenetic position in cystatin superfamily.
El-Halawany, M. S., Shibata, H., Hitomi, K., and Maki, M.
Mol. Biol. Rep. 32, 257-264 (2005)

Distinct mechanistic roles of calpain and caspase activation in neurodegeneration as revealed in mice overexpressing their specific inhibitors.
Higuchi M, Tomioka M, Takano J, Shirotani K, Iwata N, Masumoto H, Maki M, Itohara S, Saido TC.　
J Biol Chem. 280, 15229-15237 (2005)

Calpain mediates excitotoxic DNA fragmentation via mitochondrial pathways in adult brains: Evidence from calpastatin-mutant mice.
Takano J, Tomioka M, Tsubuki S, Higuchi M, Iwata N, Itohara S, Maki M, Saido TC.
J Biol Chem. 280, 16175-16184 (2005)

Overexpression of calpastatin inhibits L8 myoblast fusion.
Barnoy S, Maki M, Kosower NS.
Biochem Biophys Res Commun. 2005 Jul 8;332(3):697-701.

Identification of Rab GTPase-activating protein-like protein (RabGAPLP) as a novel Alix/AIP1-interacting protein.
Ichioka, F., Horii, M., Katoh, K., Terasawa, Y., Shibata, H., and Maki, M.
Biosci. Biotechnol. Biochem. 69, 861-865 (2005)

Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting.
Yorikawa, C., Shibata, H., Waguri, S., Hatta, K., Horii, M., Katoh, K., Kobayashi, T., Uchiyama, Y., and Maki, M.
Biochem. J. 387, 17-26 (2005)

Dictyostelium discoideum requires an Alix/AIP1 homolog, DdAlix, for morphogenesis in alkaline environments
Ohkouchi, s., Saito, H., Aruga, F., Maeda, T., Shibata, H., and Maki, M.
FEBS Lett. 579, 1745-1750 (2005)

DdAlix, an Alix/AIP1 homolog in Dictyostelium discoideum, is required for multicellular development under low Ca2+ conditions
Ohkouchi, S., El-Halawany, M. S., Aruga, F., Shibata, H., Hitomi, K., and Maki, M.
Gene 337, 131-139 (2004)

Identification of cysteine protease inhibitors that belong to cystatin family 1 in the cellular slime mold Dictyostelium discoideum.
El-Halawany MS, Ohkouchi S, Shibata H, Hitomi K, Maki M.
Biol. Chem. 385, 547-550 (2004)

CHMP4b is a major binding partner of the ALG-2-interacting protein Alix among the three CHMP4 isoforms.
katoh, K., Shibata, H., Hatta, K. and Maki, M.
Arch. Biochem. Biophys. 421, 159-165 (2004)

The penta-EF-hand protein ALG-2 interacts with a region containing PxY repeats in Alix/AIP1, which is required for the subcellular punctate distribution of the amino-terminal truncation form of Alix/AIP1.
Shibata, H., Yamada, K., Mizuno, T., Yorikawa, C., Takahashi, H., Satoh, H., Kitaura, Y., and Maki, M.
J. Biochem. 135, 117-128 (2004)

The ALG-2-interacting protein Alix associates with CHMP4b, a human homologue of yeast Snf7 that is involved in multivesicular body sorting.
Katoh, K., Shibata, H., Suzuki, H., Nara, A., Ishidoh, K., Kominami, E., Yoshimori, T., and Maki, M.
J. Biol. Chem.278, 39104-39113 (2003)

Calpain regulates enterocyte brush border actin assembly and pathogenic Escherichia coli-mediated effacement.
Potter DA, Srirangam A, Fiacco KA, Brocks D, Hawes J, Herndon C, Maki M, Acheson D, Herman IM.
J Biol Chem. 2003 Aug 8;278(32):30403-12. Epub 2003 May 22.

A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor.
Todd B, Moore D, Deivanayagam CC, Lin GD, Chattopadhyay D, Maki M, Wang KK, Narayana SV.
J Mol Biol. 2003 Apr 18;328(1):131-46.

Structures, functions and molecular evolution of the penta-EF-hand Ca 2+-binding proteins. *
Maki, M., Kitaura, Y., Satoh, H., Ohkouchi, S., and Shibata, H.
Biochim. Biophys. Acta 1600, 51-60 (2002)

The penta-EF-hand domain of ALG-2 interacts with amino-terminal domains of both annexin VII and annexinXI in a Ca2+-dependent manner.
Satoh, H., Nakano, Y., Shibata, H., and Maki, M.
Biochim. Biophys. Acta 1600, 61-67 (2002)

Both ALG-2 and peflin, penta-EF-hand (PEF) proteins, are stabilized by dimerization through their fifth EF-hand regions.
Kitaura, Y., Satoh, H., Takahashi, H., Shibata, H., and Maki, M.
Arch. Biochem. Biophys. 399, 12-18 (2002)

Peflin and ALG-2, members of the penta-EF-hand protein family, form a heterodimer that dissociates in a Ca2+-dependent manner.
Kitaura, Y., Matsumoto, S., Satoh, H., Hitomi, K., and Maki, M.
J. Biol. Chem. 276, (2001)