LOCUS SYNPUC18V 2686 bp DNA circular SYN 27-APR-1993 DEFINITION pUC18 cloning vector. ACCESSION L08752 VERSION L08752.1 GI:209211 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 (sites) AUTHORS Norrander,J., Kempe,T. and Messing,J. TITLE Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis JOURNAL Gene 26 (1), 101-106 (1983) PUBMED 6323249 REFERENCE 2 (bases 1 to 2686) AUTHORS Gilbert,W. TITLE Obtained from VecBase 3.0 JOURNAL Unpublished (1991) COMMENT These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. pUC18 - Cloning vector ENTRY PUC18 #TYPE DNA CIRCULAR TITLE pUC18 - Cloning vector DATE 17-SEP-1986 #sequence 16-DEC-1986 ACCESSION VB0025 SOURCE artificial COLLECTION ATCC 37253 REFERENCE #number 1 #authors Norrander J., Kempe T., Messing J. #journal Gene (1983) 26: 101-106 REFERENCE #number 2 #authors Pouwels P.H., Enger-Valk B.E., Brammar W.J. #book Cloning Vectors, Elsvier 1985 and supplements #comment vector I-A-iv-20 COMMENT Assembled from pUC19 and M13mp18 by F. Pfeiffer, MPI, Martinsried Revised 16-DEC-1986 by F. Pfeiffer: 2308/9 'AT' to 'TA' to match revised sequence of PBR322 The strand shown contains the beta-galactosidase mRNA sequence including the multiple cloning site of M13mp18. KEYWORDS CROSSREFERENCE #complement VecBase(3):pUC18c #parent VecBase(3):pUC12, VecBase(3):M13mp18, VecSource(3):bGal18 #brother VecBase(3):pUC19 #offspring VecBase(3):pGEM3, VecBase(3):pTZ18R, VecBase(3):pIBI24, VecBase(3):pIBI30, VecBase(3):pSP18, VecBase(3):pT7T318, VecBase(3):pT7T3A18 PARENT Features of pUC18 (2686 bp) residue source 1- 447 6002-6448 M13mp18 1- 229 1069-1297 Lac-Operon 230- 286 1- 57 polylinker of M13mp18 289- 447 1303-1461 Lac-Operon 448- 547 2351-2252 (c) pBR322 548- 684 2210-2074 (c) pBR322 685-2686 4355-2354 (c) pBR322 Conflict (cfl) and Mutations (mut): pUC18 source mut 1128 T C 3912 (c) pBR322 mut 1429 A G 3611 (c) pBR322 FEATURE 952-1740 1-789 Ap-R; b-lactamase POLYLINKER EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-PstI-SphI-HindIII SELECTION #resistance Ap #indicator beta-galactosidase SUMMARY pUC18 #length 2686 #checksum 5464. FEATURES Location/Qualifiers source 1..2686 /organism="synthetic construct" /mol_type="genomic DNA" /db_xref="taxon:32630" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct 241 cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca ctggccgtcg 301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt 481 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt 541 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 601 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 661 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg 721 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc 781 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 841 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 901 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 961 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 1021 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 1081 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc 1141 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 1201 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 1261 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 1321 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 1381 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 1441 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 1501 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 1561 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 1621 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 1681 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 1741 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 1801 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 1861 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 1921 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 1981 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 2041 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 2101 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 2161 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 2221 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 2281 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 2341 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 2401 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 2461 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 2521 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 2581 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 2641 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga //