Department of Biological Mechanisms and Functions

Division of Biodynamics


Prof. MAESHIMA, Masayoshi D. Agr. maeshima@
Asst. Prof. NAKANISHI, Youichi D. Agr. nakanish@
Des. Asst. Prof. SEGAMI, Shoji D. Agr. segami@

Our research focuses on the molecular structure, transport mechanism, biochemical regulation of the membrane transport systems, and signal-transduction proteins associated with membrane in plant cells, with the objective of elucidating their structure-function relationship and physiological roles. At present we investigate the vacuolar H+-pyrophosphatase, H+-ATPase, Zn2+/H+ antiporter, aquaporins, metal transporters, and novel Ca2+-binding proteins. This information will introduce a higher order of molecular aspect in cell dynamics of plants.

Recent Publications:

  1. Fujiwara, T., Mori, H., Kawachi, M. and Maeshima, M. (2015) Identification of a unique CDF member containing the long N-terminal region in Arabidopsis thaliana. FEBS J., 282, 1965-1979.
  2. Segami, S., Makino, S., Miyake, A., Asaoka, M. and Maeshima, M. (2014) Dynamics of vacuoles and H+-pyrophosphatase visualized by monomeric green fluorescent protein in Arabidopsis: Artifactual bulbs and native intravacuolar spherical structures.  Plant Cell, 26, 3416-3434.
  3. Tanaka, N., Kato, M., Tomioka, R., Kurata, R., Fukao, Y., Aoyama, T. and Maeshima, M. (2014) Characteristics of a root hair-less line of Arabidopsis thaliana under physiological stresses.  J. Exp. Botany, 65, 1497-1512.
  4. Bak, G., Lee, E.J., Lee, Y., Kato, M., Segami, S., Heven, S., Maeshima, M., Hwang, J.U. and Lee, Y. (2013) A role of phosphatidylinositol 3,5-bisphosphate in vacuolar structure change in guard cells of closing stomata.  Plant Cell, 25, 2202–2216.
  5. Kato, M., Aoyama, T. and Maeshima, M. (2013) A Ca2+-binding protein PCaP2 located on the plasma membrane is involved in root hair development as a possible signal transducer.  Plant J., 74, 690-700.
  6. Nakanishi, Y., Iida, S., Ueoka-Nakanishi, H., Niimi, T., Tomioka, R. and Maeshima, M. (2013) Exploring dynamics of molybdate in living animal cells by a genetically encoded FRET nanosensor.  PLoS One, 8, e58175.
  7. Kawachi, M., Kobae, Y., Kogawa, S., Mimura, T., Krämer, U. and Maeshima, M. (2012) Amino acid screening based on structural modeling identifies critical residues for the function, ion selectivity and structure of Arabidopsis MTP1. FEBS J., 279, 2339-2356.


  FAX: +81-52-789-4283
Assoc. Prof. YOSHIOKA, Hirofumi D. Agr. hyoshiok@

The main focus of our research is to understand the molecular mechanisms of plant immune system in plant-pathogen interactions. One of our goals is to produce genetically engineered plants showing disease resistance in such a way as to reduce the use of environmentally damaging fungicides.

Current themes:

  1. Regulatory mechanisms of NADPH oxidase and role of ROS in plant immunity.
  2. Regulatory mechanisms of NO production and its role in plant immunity.
  3. Downstream signaling pathway of defense-related MAP kinase cascades.
  4. Production of transgenic potato plants showing resistance to Phytophthora infestans.
  5. Signaling pathway of host-selective toxin produced by a plant pathogenic fungus.
  6. Defense mechanisms between herbivore and plant interactions.

Selected publications:

  1. Kobayashi, M., Ohura, I., Kawakita, K., Yokota, N., Fujiwara, M., Shimamoto, K., Doke, N. and Yoshioka, H. (2007) Calcium-dependent protein kinases regulate production of reactive oxygen species by potato NADPH oxidase. Plant Cell 19, 1065-1080.
  2. Asai, S., Ohta, K. and Yoshioka, H. (2008) MAPK signaling regulates nitric oxide and NADPH oxidase-dependent oxidative bursts in Nicotiana benthamiana. Plant Cell 20, 1390-1406.
  3. Ishihama, N., Yamada, R., Yoshioka, M., Katou, S. and Yoshioka, H. (2011) Phosphorylation of the Nicotiana benthamiana WRKY8 transcription factor by MAPK functions in the defense response. Plant Cell 23, 1153-1170.
  4. Kobayashi, M., Yoshioka, M., Asai, S., Nomura, H., Kuchimura, K., Mori, H., Doke, N. and Yoshioka, H. (2012) StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. New Phytol. 196, 223-237.
  5. Nomura, H., Komori, T., Uemura, S., Kanda, Y., Shimotani, K., Nakai, K., Furuichi, T., Takebayashi, K., Sugimoto, T., Sano, S., Suwastika, I.N., Fukusaki, E., Yoshioka, H., Nakahira, Y. and Shiina, T. (2012) Chloroplast-mediated activation of plant immune signalling in Arabidopsis. Nature Commun. 3, 926.
  6. Asai, S., Ichikawa, T., Nomura, H., Kobayashi, M., Kamiyoshihara, Y., Mori, H., Kadota, Y., Zipfel, C., Jones, J.D.G. and Yoshioka, H. (2013) The variable domain of a plant calcium-dependent protein kinase (CDPK) confers subcellular localization and substrate recognition for NADPH oxidase. J. Biol. Chem. 288, 14332-14340.
  7. Yamaguchi, K., Yamada, K., Ishikawa, K., Yoshimura, S., Hayashi, N., Uchihashi, K., Ishihama, N., Kishi-Kaboshi, M., Takahashi, A., Tsuge, S., Ochiai, H., Tada, Y., Shimamoto, K., Yoshioka, H. and Kawasaki, T. (2013) A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity. Cell Host Microbe 13, 347-357.
  8. Mase, K., Ishihama, N., Mori, H., Takahashi, H., Kaminaka, H., Kodama, M. and Yoshioka, H. (2013) Ethylene responsive AP2/ERF transcription factor MACD1 participates in phytotoxin-triggered programmed cell death. Mol. Plant-Microbe Interact. 26, 868-879.
  9. Le Roux, C., Huet, G., Jauneau, A., Camborde, L., Trémousaygue, D., Kraut, A., Zhou, B., Levaillant, M., Adachi, H., Yoshioka, H., Raffaele, S., Berthomé, R., Couté, Y., Parker, J.E. and Deslandes, L. (2015) A receptor pair with an integrated decoy converts pathogen disabling of transcription factors to immunity. Cell 161, 1074-1088.
  10. Adachi, H., Nakano, T., Miyagawa, N., Ishihama, N., Yoshioka, M., Katou, Y., Yaeno, T., Shirasu, K. and Yoshioka, H. (2015) WRKY transcription factors phosphorylated by MAPK regulate a plant immune NADPH oxidase in Nicotiana benthamiana. Plant Cell 27, 2645-2663.